First Report of Cucurbit Chlorotic Yellows Virus Infecting Cucumber in South Korea

HomePlant DiseaseVol. 105, No. 6First Report of Cucurbit Chlorotic Yellows Virus Infecting Cucumber in South Korea Previous DISEASE NOTES OPENOpen Access licenseFirst KoreaH.-R. Kwak, H.-S. Byun, Choi, J.-W. Han, C.-S. Kim, W. M. Wintermantel, J.-E. and KimH.-R. Kwakhttps://orcid.org/0000-0002-1852-9776Rural Development Administration, National Institute Agricultural Science, Wanju 55365, KoreaSearch for more papers by this author, ByunRural ChoiRural HanChungcheongbuk-do Research Extension Services, Cheongju 28130, KimRural Highland Agriculture Institute, Pyeongchang 25342, Wintermantelhttps://orcid.org/0000-0001-9562-5262United States Department Agriculture, Service, Salinas, CA, U.S.A.Search KimDepartment Plant Medicine, Chungbuk University, 28644, Kim†Corresponding author: Kim; E-mail Address: [email protected] author AffiliationsAuthors Affiliations H.-R. Kwak1 Byun1 Choi1 Han2 Kim3 Wintermantel4 Kim5 † 1Rural 2Chungcheongbuk-do 3Rural 4United U.S.A. 5Department Published Online:22 Apr 2021https://doi.org/10.1094/PDIS-10-20-2254-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleIn October 2018, cucumber plants showing yellowing chlorotic mottle symptoms were observed a greenhouse Chungbuk, Korea. The similar those caused cucurbit aphid-borne yellows virus (CABYV), which has been detected on the region since it was reported melon 2015 (Lee et al. 2015). To identify potential agents causing these symptoms, 28 samples from symptomatic leaves fruit subjected total RNA extraction using Prep Kit (Biocubesystem, Korea). Reverse transcription polymerase chain reaction (RT-PCR) performed CABYV-specific primers protocols (Kwak 2018). CABYV 17 samples, whereas 11 tested negative. cause RT-PCR specific (CCYV) yellow stunting disorder (CYSDV) (Wintermantel 2019). Eight positive CCYV-specific primers, seven infected with only CCYV one contained mixed infection CCYV. None CYSDV. expected 373-nt amplicons bidirectionally sequenced, BLASTn analysis showed that nucleotide sequences shared 98 100% identity isolates East Asia, including NC0180174 Japan. Two pairs amplification complete coat protein RNA-dependent (RdRp) genes 2019) used amplify 753-bp 1,517-bp RdRp genes, respectively. Amplicons sizes obtained single ligated into pGEM T-Easy vector (Promega, WI). Three clones each amplicon sequenced aligned Geneious Prime found have identical (GenBank accession nos. MW033300 MW033301). CP demonstrated 99% amino acid respective proteins This study documents first report Since Japan, many other countries Middle East, Southern Europe, North Africa, recently America. become serious threat production crops Korea, particularly due increasing prevalence whitefly, Bemisia tabaci, systems. It will be important continue monitoring determine alternate hosts manage prevent further spread Korea.The author(s) declare no conflict interest.References:Kwak, H.-R., 2018. Pathol. J. 34:532. Crossref, ISI, Google ScholarLee, H. J., 2015. Kor. Hortic. Sci. Technol. 33:210. ScholarWintermantel, M., 2019. Dis. 103:778. https://doi.org/10.1094/PDIS-08-18-1390-PDN Link, ScholarH.-R. Kwak Han contributed equally work.The interest.Funding: research supported grants Agenda Program funded Rural Administration (PJ01530804).DetailsFiguresLiterature CitedRelated Vol. 6 June 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionDetail cassava mosaic disease as field (K. Chittarath al.). Photo credit: K. Chittarath. Bird’s-eye lesion tomato cv. Ailsa Craig (F. C. Peritore-Galve F. Peritore-Galve. Metrics Downloaded 575 times Article History Issue Date: 19 Oct 2021Published: 22 2021First Look: Jan 2021Accepted: 5 2021 Page: 1862 Information© American Phytopathological SocietyFundingRural AdministrationGrant/Award Number: PJ01530804Keywordscriniviruscucurbitavirus detectionwhiteflyCucumis sativusThe interest.